Every year, 10 million people fall ill with tuberculosis (TB). Despite being a preventable and curable disease, 1.5 million people die from TB each year. TB is the leading cause of death of people with HIV and a major contributor to antimicrobial resistance. Most of the people who fall ill with TB live in low- and middle-income countries, but TB is present all over the world. This meeting aims to bring together microbiologists, cell biologists, immunologists, and clinicians to collectively tackle the public health challenge that TB presents worldwide. The meeting will showcase new technologies and approaches that will allow us to understand the disease across scales, from bacteria to the human host and their interactions. The meeting will engage and feature the next generation of TB researchers to drive the field forward and foster cross-disciplinary insights and collaborations. TB mostly affects the Global South, but its research is poorly represented in scientific meetings. We aim to enable early-career researchers from the Global South to exchange ideas and discuss research approaches and resources that are needed for future research with leading experts in the TB field.