Despite the availability of effective treatment and prevention strategies, the HIV pandemic continues, with approximately 1.7 million new infections annually. More effective prevention strategies are needed. The goal of this meeting is to assemble experts on the biology of HIV transmission and emerging prevention methods - including vaccines and PrEP - to discuss the current state-of-the-art in HIV prevention. Specific topics of discussion will include (2) germline vaccination strategies, (2) updates on the vaccine and PrEP efficacy studies, (3) challenges and opportunities of developing novel strategies in an era of increasingly effective prevention methods, particularly long-acting PrEP (cabotegravir) and the dapivirine ring, and (4) next generation vaccine and PrEP approaches. The role that HIV prevention resources and advances have had on the COVID-19 prevention and HIV cure research agendas will also be discussed, with the hope that these three largely complementary areas of investigation will remain highly synergistic.