Genome editing refers to the use of programmable DNA-targeting platforms to manipulate genetic and epigenetic information in living cells and organisms. Genome editing has been recently democratized by the development of RNA-guided endonucleases repurposed from CRISPR-Cas microbial defense systems against viruses or plasmids. Whereas novel tools such as Cpf1 and RNA-guided deaminases are being developed to expand the scope of genome editing, old tools such as ZFNs and TALENs are likely to undergo innovations as well. These tools enable genome-wide genetic screens in cell lines and genetic studies in both model and non-model organisms. Furthermore, genome editing holds great promise for broad applications in medicine and biotechnology, raising public interest and concerns. Yet genome editing is still limited by many factors such as inefficient delivery in vivo, off-target effects, and unwanted, alternative DNA repair. This conference offers opportunities to learn about new developments, advancements and applications in the field.