Keystone Symposia | Scientific Conferences on Biomedical and Life Science Topics

Cell Biology of Virus Entry, Replication and Pathogenesis (B5)

Organizer(s): Margaret Kielian, Peter Sarnow, Terence S. Dermody and Paul D. Bieniasz
February 16 - 21, 2010
Sagebrush Inn and Conference Center · Taos, New Mexico
Abstract Deadline: October 19, 2009
Late Abstract Deadline: November 16, 2009
Scholarship Deadline: October 19, 2009
Early Registration Deadline: December 16, 2009

Sponsored by Santaris Pharma A/S

This meeting took place in the 2010 season.

For a complete list of the meetings for the upcoming/current season,
see our meeting list, or search for a meeting.

Summary of Meeting
The Keystone Symposia meeting on the Cell Biology of Virus Entry, Replication and Pathogenesis emphasizes key aspects of virus infection pathways and cellular responses. A central goal is the identification of critical virus-cell crosstalk during these processes. Rather than dividing viruses into separate “categories” such as positive-sense RNA viruses and DNA viruses, the meeting highlights common aspects of virus lifecycles among different virus groups. The multi-disciplinary nature of the proposed meeting is important in bringing together investigators using structural, molecular, cell biological, immunological and epidemiological methods. This emphasis on shared themes and multiple experimental approaches will continue to be critical to future advances in virology. While there has been spectacular recent progress in our understanding of virus lifecycles, we are still very far from being able to design antiviral strategies, and unexpected novel aspects of virus cell biology are constantly being discovered. Plenary sessions will cover the most important aspects of virus interactions with cells. Day 1 will include cutting-edge structural virology studies and imaging methods to follow single virus particles during entry. Day 2 will focus on the entry mechanisms of enveloped and non-enveloped viruses and the roles of cellular proteins in virus infection. Day 3 will cover viral and cellular aspects of virus replication and cellular antiviral responses. The last day will focus on the important areas of virus assembly and pathogenesis. Late-breaking exciting developments in this fast-moving field will be incorporated by short presentations and afternoon workshop sessions drawn from the submitted abstracts.

Tuesday, February 16
3:00 - 7:30 PM	Registration	Chamisa Lobby
6:30 - 7:30 PM	Refreshments	Chamisa Lobby
7:30 - 8:30 PM	Keynote Address	Chamisa Ballroom 1
	Francis V. Chisari, The Scripps Research Institute Induction and Evasion of the Innate Host Response to HCV


8:30 - 8:45 PM	Orientation for New Attendees and New Investigators Keystone Symposia's Chief Scientific Officer, Dr. Andy Robertson, to lead discussion in a "What to Expect during your Attendance" for interested delegates.	Chamisa Ballroom 1
Wednesday, February 17
7:00 - 8:00 AM	Breakfast	Los Vaqueros
8:00 - 11:15 AM	Advances in Understanding Virus Structure and Function	Chamisa Ballroom 1
	Margaret Kielian, Albert Einstein College of Medicine Virus-Membrane Fusion: Mechanisms of the Alphavirus and Flavivirus Fusion Proteins
	* Kay Grünewald, Wellcome Trust Centre for Human Genetics Probing Membrane Interactions of Herpesvirus Glycoproteins by Cryo Electron Tomography
	* Erica Ollmann Saphire, The Scripps Research Institute Crystal Structures Reveal a Shared Immunological Solution for Neutralizing Ebolaviruses
	Richard J. Kuhn, Purdue University Dengue Virus Structure and Function during Assembly, Maturation and Entry
	Manidipa Banerjee, The Scripps Research Institute Short Talk: Entry of a Non-Enveloped Virus Depends on Metal-Mediated pH Control of the Delivery of a Lytic Peptide to the Endosomal Membrane
	Gaya Amarasinghe, Iowa State University Short Talk: Structural Basis for Innate Immune Evasion by Ebola VP35
9:20 - 9:40 AM	Coffee Break	Chamisa Lobby
11:15 AM -	On Own for Lunch and Recreation
11:15 AM - 1:00 PM	Poster Setup	Chamisa Ballroom 2
1:00 - 10:00 PM	Poster Viewing	Chamisa Ballroom 2
2:30 - 4:30 PM	Workshop 1: Virus Entry and Assembly	Chamisa Ballroom 1
	* Colin R. Parrish, Cornell University
	* Ted C. Pierson, NIAID, National Institutes of Health
	Christopher A. Davis, University of Birmingham Claudin Association with CD81 Defines Hepatitis C Virus Entry
	Julia Bitzegeio, TWINCORE Adaptation of Hepatitis C Virus to Mouse CD81 Permits Infection of Mouse Cells in the Absence of Human Factors
	Walther Mothes, Yale University School of Medicine Mechanism of Cell-to-Cell Transmission of Retroviruses
	Jason Laliberte, NIAID, National Institutes of Health The Membrane Fusion Step of Vaccinia Virus Entry is Cooperatively Mediated by Twelve Viral Membrane Proteins
	Kai Xu, Memorial Sloan Kettering Cancer Center Structural Studies on the Henipavirus G Attachment Glycoprotein and a Cross-Reactive Neutralizing Human Monoclonal Antibody
	Rebecca Ellis Dutch, University of Kentucky Paramyxovirus Fusion Protein Transmembrane Domain Interactions: Potential Roles in Protein Folding, Membrane Fusion and Intracellular Trafficking
	Joshua Matthew Costin, Florida Gulf Coast University Peptide-Induced Ejection of the Dengue Virus Genome
	Andrew M. Lee, The Scripps Research Institute Probing Arenavirus Fusion Using Unique Small Molecules

4:30 - 5:00 PM	Coffee Available	Chamisa Lobby
5:00 - 7:00 PM	Approaches to Visualizing Virus Entry	Chamisa Ballroom 1
	* Robert W. Doms, University of Pennsylvania School of Medicine
	Xiaowei Zhuang, Harvard University Zooming in on Virus-Cell Interactions – By Single Particle Tracking and Sub-Diffraction Limit Imaging
	Thomas J. Hope, Northwestern University, Feinberg School of Medicine Single Virus Tracking of HIV in vivo
	Sean P. Whelan, Harvard Medical School Approaches to Characterizing the Entry of Vesicular Stomatitis Virus into Host Cells
	Carolyn B. Coyne, University of Pittsburgh Short Talk: Coxsackievirus B3 Internalization into Polarized Endothelial Cells Requires DAF-Mediated Release of ER-Derived Calcium Stores
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Chamisa Ballroom 2
7:30 - 10:00 PM	Poster Session 1	Chamisa Ballroom 2
Thursday, February 18
7:00 - 8:00 AM	Breakfast	Los Vaqueros
8:00 - 11:15 AM	Virus Entry Pathways	Chamisa Ballroom 1
	* Glen R. Nemerow, The Scripps Research Institute
	Terence S. Dermody, Vanderbilt University School of Medicine Reovirus Internalization and Proteolytic Disassembly
	Matthew J. Evans, Mount Sinai School of Medicine Hepatitis C Virus Cell Entry Factors And Pathways
	Kartik Chandran, Albert Einstein College Of Medicine Proteolysis and the Membrane Fusion Mechanism of Ebolavirus
	* Billy Tsai, University of Michigan Medical School Polyomavirus Entry Pathway
	Daniel C. DiMaio, Yale University School of Medicine Short Talk: Cellular DNAJ Proteins are Required for Efficient SV40 Infection
	Megan Shaw, Mount Sinai School of Medicine Short Talk: Human Host Factors Required for Influenza Virus Replication
9:20 - 9:40 AM	Coffee Break	Chamisa Lobby
11:15 AM -	On Own for Lunch and Recreation
11:15 AM - 1:00 PM	Poster Setup	Chamisa Ballroom 2
1:00 - 10:00 PM	Poster Viewing	Chamisa Ballroom 2
4:30 - 5:00 PM	Coffee Available	Chamisa Lobby
5:00 - 7:00 PM	Cellular Genes and Virus Infection	Chamisa Ballroom 1
	Karla Kirkegaard, Stanford University School of Medicine Autophagy and Virus Infection
	* Sara R. Cherry, University of Pennsylvania School of Medicine Identification of Factors Involved in Virus Replication using RNAi Screens
	* Susana Lopez, Instituto de Biotecnologia Rotavirus Strategies to Control the Cell Protein Synthesis Apparatus
	Matthew D. Weitzman, The Salk Institute Short Talk: A Viral Ubiquitin Ligase Targets RNF8 and RNF168 to Modulate Histone Ubiquitination and the Cellular DNA Damage Response
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Chamisa Ballroom 2
7:30 - 10:00 PM	Poster Session 2	Chamisa Ballroom 2
Friday, February 19
7:00 - 8:00 AM	Breakfast	Los Vaqueros
8:00 - 11:15 AM	Virus Replication	Chamisa Ballroom 1
	* Robert F. Kalejta, University of Wisconsin-Madison
	Peter Sarnow, Stanford University School of Medicine Modulation of Hepatitis C Virus RNA Abundance by MicroRNA 122 and Residents of Processing Bodies

	Ian J. Mohr, New York University School of Medicine Regulation of Translation during Virus Infection
	Peter D. Nagy, University of Kentucky A Systems Biology Approach to Dissect the Roles of Host Proteins in Tombusvirus RNA Replication
	Andrea Gamarnik, Fundación Instituto Leloir Dengue Virus Capsid Protein Localization in Lipid Droplets and its Interplay with Viral RNA Synthesis
	Sunnie R. Thompson, University of Alabama at Birmingham Short Talk: A Genetic Approach to Understanding how Viral Internal Ribosome Sites Recruit Host Ribosomes
	Oren Kobiler, Princeton University Short Talk: A “Brainbow” Herpesvirus, Able to Switch Fluorophores, Suggests a Limited Number of Viral Genomes Express and Replicate in a Newly Infected Cell
9:20 - 9:40 AM	Coffee Break	Chamisa Lobby
11:15 AM -	On Own for Lunch and Recreation
11:15 AM - 1:00 PM	Poster Setup	Chamisa Ballroom 2
1:00 - 10:00 PM	Poster Viewing	Chamisa Ballroom 2
2:30 - 4:30 PM	Workshop 2: Viral Pathogenesis and Host Interactions	Chamisa Ballroom 1
	* Jaisri R. Lingappa, University of Washington
	* Daniel C. DiMaio, Yale University School of Medicine
	Daniel Popkin, The Scripps Research Institute Mapping the Chronic Viral Infection Resistome
	Jan E. Carette, Whitehead Institute for Biomedical Research Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens
	Shane D. Trask, NIAID, National Institutes of Health Engineering Rotavirus Reassortants by Reverse Genetics
	Ivo Lorenz, International AIDS Vaccine Initiative Insertion of the HIV-1 gp41 Epitopes 2F5 and 4E10 into the Membrane-Proximal Region of the Vesicular Stomatitis Virus Glycoprotein
	Svetlana M. Atasheva, University of Alabama at Birmingham Venezuelan Equine Encephalitis Virus Capsid Forms a Tetrameric Complex with CRM1 and Importin-alphabeta that Obstructs Nuclear Pore Complex Function
	Sarah Cohen, University of British Columbia Caspase-Mediated Nuclear Envelope Disruption is Important for the Parvovirus Replication Cycle
	Eric Chi-Wang Yu, Burnham Institute for Medical Research A Bcl-2-like, Vaccinia Viral Protein, F1L is a Caspase-9 Inhibitor
	Andrew A. Mehle, University of California, Berkeley Adaptive Strategies of the Influenza Virus Polymerase for Replication in Humans
4:30 - 5:00 PM	Coffee Available	Chamisa Lobby
5:00 - 7:00 PM	Immune Responses and Viral Evasion	Chamisa Ballroom 1
	* Herbert (Skip) W. Virgin IV, Washington University School of Medicine
	Harmit Singh Malik, Fred Hutchinson Cancer Research Center Molecular Arms Races between Viruses and Primate Genomes
	Beth Levine, University of Texas Southwestern Medical Center Autophagy in Mammalian Alphavirus Host Defense
	Shou-Wei Ding, University of California, Riverside siRNAs and piRNAs in Viral Immunity
	Julie K. Pfeiffer, University of Texas Southwestern Medical Center Short Talk: The Gut Microbiota Influence Enteric Virus Shedding, Replication, and Pathogenesis
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Chamisa Ballroom 2
7:30 - 10:00 PM	Poster Session 3	Chamisa Ballroom 2
Saturday, February 20
7:00 - 8:00 AM	Breakfast	Los Vaqueros
8:00 - 11:15 AM	Virus Assembly and Exit	Chamisa Ballroom 1
	* Heinrich Gottlinger, University of Massachusetts Medical School
	Paul D. Bieniasz, Aaron Diamond AIDS Research Center Retrovirus Particle Assembly and Release
	Juan Martin-Serrano, King's College London School of Medicine Parallels between Viral Budding and Cytokinesis
	Thomas C. Mettenleiter, Friedrich-Loeffler-Institut Assembly and Exit Pathway of Herpesvirus
	Anette Schneemann, The Scripps Research Institute Molecular and Cell Biological Requirements for Specific Packaging of a Multipartite Viral Genome
	Jeremy Rossman, Northwestern University Short Talk: The Influenza Virus M2 Ion Channel Protein Mediates the ESCRT-Independent Budding of Filamentous Virions
	Brett Lindenbach, Yale University School of Medicine Short Talk: Hepatitis C Virus NS2 Protein Mediates Distinct, Early Steps in Virus Particle Assembly
9:20 - 9:40 AM	Coffee Break	Chamisa Lobby
11:15 AM -	On Own for Lunch and Recreation
4:30 - 5:00 PM	Coffee Available	Chamisa Lobby
5:00 - 7:00 PM	Viral Pathogenesis	Chamisa Ballroom 1
	* Barbara Sherry, North Carolina State University

	Nancy C. Reich, Stony Brook University Activation of Cellular Transcription Factors in Innate Host Defense
	Adolfo Garcia-Sastre, Mount Sinai School of Medicine Pathogenesis of Influenza Virus
	Raul Andino, University of California, San Francisco Short Talk: The Structure of the Viral Quasispecies and its Evolvability
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Chamisa Ballroom 2
8:00 - 11:00 PM	Entertainment	Chamisa Ballroom 2
Sunday, February 21
	Departure

*Session Chair †Speaker invited, not yet responded.